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progen 61055現(xiàn)貨——上海起發(fā)

更新時(shí)間：2024-03-22 點(diǎn)擊次數(shù)：400

progen 61055現(xiàn)貨——上海起發(fā)

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

抗AAV2（完整顆粒）小鼠單克隆抗體

關(guān)鍵功能

純化、凍干

小鼠單克隆抗體

適用于斑點(diǎn)印跡、ELISA、ICC和中和測(cè)定

與AAV2、AAV2 7m8和AAV3完整顆粒反應(yīng)

同種型：IgG3

Quantity	50 µg
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG3
Conjugate	Unconjugated
Application	Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification	Affinity chromatography
Reactivity	AAV2, AAV2 7m8, AAV3
No reactivity	AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74
Storage before reconstitution	2-8°C until indicated expiry date
Storage after reconstitution	2-8°C
Intended use	Research use only
Clone	A20
Immunogen	AAV2 capsids
Concentration	50 µg/ml after reconstitution with 1 ml dist. water
Formulation	Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Binding affinity	KD value (AAV2) = 2.6E-11 M KD value (AAV3) = <1.0E-12 M
Synonym	Adeno-associated virus 2; AAV-2

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Affinity Chromatography	Assay dependent
Dot Blot	1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA	Assay dependent
Immunoprecipitation (IP)	1:5
Neutralization Assay	EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) - assay dependent

用于表征感染的不同階段，對(duì)分析AAV2組裝過(guò)程非常有用。A20與AAV2和AAV3、空衣殼和全衣殼特異性反應(yīng)。識(shí)別組裝衣殼的構(gòu)象表位，不存在于變性衣殼蛋白和天然但未組裝的衣殼蛋白中。該抗體不能用于免疫印跡。

表位定位實(shí)驗(yàn)（Wobus等人，2000）確定了四個(gè)免疫反應(yīng)（不連續(xù)）區(qū)域。主要反應(yīng)歸因于AAV2衣殼的AA369至aa378序列。該抗體也可用于中和實(shí)驗(yàn)。

Wobus，C.E.等。針對(duì)腺相關(guān)病毒2型（AAV-2）衣殼的單克隆抗體：參與AAV-2細(xì)胞相互作用和中和AAV-2感染的衣殼結(jié)構(gòu)域的表位定位和鑒定。J.維羅爾。74, 9281-93 (2000).

Moskalenko，M.等。人類抗腺相關(guān)病毒2型中和抗體的表位定位：對(duì)Ge的意義